Invitrogen Anti-Firefly luciferase Monoclonal (Luci 21 1-107), Catalog # MA1-16880. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin)

Luciferase Antibody Staining For Flow

  • Protocol: Cell Surface Antibody Staining for Flow ...
  • Luciferase Antibody Fluorescein Conjugated 200-102-150
  • Rapid obtention of stable, bioluminescent tumor cell lines ...
  • Luciferase Assay - AbVideo™ - Support - Abnova
  • Protocol: Cell Surface Antibody Staining for Flow ...

    Extracellular Staining for Flow Cytometry There are many protocols for staining cells for flow cytometry. Protocols may to need be optimized for different cell types, targets, or applications. This is our basic protocol for extracellular staining of cell surface epitopes in suspension cells for flow cytometry. For intracellular staining, see our Protocol: Intracellular Antibody Staining for ... Abeomics provides Caspase-3, DNMT1, B7-H4, PD-L1 Monoclonal antibodies, ELISA kits, CTLA4 stable cell lines, recombinant proteins, TCF, TLR, Stat reporter cell lines, inhibitors and ligands. ABEOMICS develops Caspase-3, B7-H4, Dnmt1 Antibodies, PD-L1, TLR, TCF Stat Reporter Cell Line CTLA4, PD-1 Stable Cell lines, Luciferase Assay Kit, PD-L1 Pre-Coated ELISA Kits, Recombinant Antibodies ...

    Santa Cruz Biotechnology

    SIGN IN WITH SOCIAL ACCOUNT. Please sign in using your account on one of the following social networks. Flow cytometric analysis of HEK293 cells expressing Firefly Luciferase, staining Firefly Luciferase with ab16466. Cells were fixed with paraformaldehyde at room temperature for 20 min, rinsed twice with permeabilization buffer (PBS, 1% FBS, 0.1% saponin), and incubated with primary antibody. Prepare the luciferase assay solution (see the Table of Materials) by diluting the Nluc substrate 1:50 with the dilution buffer. In a white flat-bottom 96-well microplate for luciferase assays, mix 25 µL of luciferase assay solution with 10 to 25 µL of the collected tissue culture supernatant samples.

    Luciferase expression and bioluminescence does not affect ...

    Luciferase expression and bioluminescence does not affect tumor cell growth in vitro or in vivo. ... each clone was analyzed by flow cytometry to ensure GFP expression remained stable after several weeks in culture ... (Cat. # 9664). A secondary antibody conjugated to alkaline phosphatase followed by chromogen staining (Fast Red) was used to ... Fusion protein was detected using murine anti-Renilla luciferase monoclonal antibody (Chemicon, Temecula, CA) followed by goat anti-mouse antibody conjugated to phycoerythrin (Jackson Immunoresearch, West Grove, PA) on a MoFlo flow cytometer (Cytomation, Fort Collins, CO). Murine anti-CEA T84.1 antibody was used as a positive control.

    Anti-Firefly Luciferase antibody [Luci17] (ab16466) | Abcam

    Flow cytometric analysis of HEK293 cells expressing Firefly Luciferase, staining Firefly Luciferase with ab16466. Cells were fixed with paraformaldehyde at room temperature for 20 min, rinsed twice with permeabilization buffer (PBS, 1% FBS, 0.1% saponin), and incubated with primary antibody. Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. produced using ATP-dependent luciferase. Other ATP assays use the ATP-dependent ... DNA-staining dyes are commonly used in flow cytometry to measure the DNA content in cell populations and assay for cell cycle state. Propidium iodide is the mostly ... Edu staining is consistent with further antibody staining, unlike the harsher BrdU protocol.

    Anti-Firefly Luciferase 抗体 [Luci17] (ab16466) | アブカム

    Flow cytometric analysis of HEK293 cells expressing Firefly Luciferase, staining Firefly Luciferase with ab16466. Cells were fixed with paraformaldehyde at room temperature for 20 min, rinsed twice with permeabilization buffer (PBS, 1% FBS, 0.1% saponin), and incubated with primary antibody. FACS: sorting cells based on flow cytometry data The terms flow cytometry and fluorescence-activated cell sorting (FACS) are often used interchangeably. In practice, there are differences between the two methods. FACS is a derivative of flow cytometry that adds an exceptional degree of functionality.

    Luciferase Antibody Fluorescein Conjugated 200-102-150

    Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Development of a luciferase reporter Jurkat cell line under the control of endogenous interleukin-2 promoter ... cell surface expression of GITR was confirmed by staining cells with fluorescence-labeled anti-GITR antibody followed by flow cytometery ... in the presence of various concentrations of anti-GITR antibody. Luciferase activities were ...

    A novel luciferase-based assay for the detection of ...

    A novel luciferase-based assay for the detection of Chimeric Antigen Receptors ... In contrast to Protein-L or anti-idiotype antibody based flow-cytometry assays, the Topanga assay is an assay of ... Stainingprotocol’ ’ Cells:’4T1’mouse’mammary’carcinoma’cell’line’transfected’with’luciferase’ ’ ’ 1.Placed5x’1054T1cellsina’96well ...

    Luciferase Antibody (NB100-1677): Novus Biologicals

    FAQs for Luciferase Antibody (NB100-1677). (Showing 1 - 2 of 2 FAQs). One of our customers would like additional information about your goat anti-luciferase (catalog #NB100-1677). They want to use this antibody for immunohistochemistry of paraffin-embedded sections, and would like to know if you recommend an antigen retrieval step? The human embryonic stem cell line NERCe002-A-2 was generated by transduction of NERCe002-A cells with an expression vector carrying the luciferase gene. The stem cells labelled with luciferase can be transplanted into animals and detected by the bioluminescence imaging technology. Article A Bioluminescence Resonance Energy Transfer-Based Approach for Determining Antibody-Receptor Occupancy In Vivo Yu Tang,1 Kshitij Parag-Sharma,2 Antonio L. Amelio,3,4 and Yanguang Cao1,4,5,* SUMMARY Elucidating receptor occupancy (RO) of monoclonal antibodies (mAbs) is a crucial step in character-

    Luciferase Antibody | SCBT - Santa Cruz Biotechnology

    Select Luciferase antibodies from monoclonal antibodies listed below. View detailed Luciferase antibody specifications by linking to the specific product blocks. Select appropriate Luciferase antibodies for your research by isotype, epitope, applications and species reactivity. To the left of the product names in the table below, Development of a cytotoxicity assay based on transient expression of different luciferases in cytosol. Gaussia luciferase (Gluc; 185 aa, 19.9 kDa) is one of the smallest luciferase known and is ... Can someone suggest an antibody for immunocytochemistry and flow cytometry? ... Anti-Luciferase antibody from abcam is very unspecific. ... I have read about the possibility of detection of ...

    Rapid obtention of stable, bioluminescent tumor cell lines ...

    However, the intact fusion protein was clearly predominant with anti-luciferase as well as anti-CD2 staining. Finally surface CD2 staining and flow cytometry analysis was performed after at least 4 weeks of continuous growth (Figure (Figure7). 7). Massive and homogeneous surface expression of CD2 was recorded for transfected Daudi and Hela cells. Firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability: ... Proliferating cells in the eye of E4 chick embryo using BrdU staining B. Anti-BrdU antibody validation using Camptothecin. ... The assays are available in various formats for microscopy imaging, flow cytometry, high throughput screening ...

    Firefly luciferase Antibody (MA1-12556)

    Invitrogen Anti-Firefly luciferase Monoclonal (Luci17), Catalog # MA1-12556. Tested in Western Blot (WB) and Immunohistochemistry (Frozen) (IHC (F)) applications. This antibody reacts with Luciferase samples. Supplied as 100 µg purified antibody (1 mg/mL). The Firefly luciferase is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis endemic to Yunnan province, China. It has been modified to provide enhanced stability compared to the normal phenotype of Photinus pyralis as wel Recombinant Mouse Anti-Gaussia luciferase Antibody (GL-11-6) This product is a mouse monoclonal antibody targeting gaussia luciferase. This antibody has the inhibitory activity of the luminescence function of Gaussia luciferase.

    Luciferase Antibody (Luci 21 1-107) [PE/Cy7] (NB600 ...

    Mouse Monoclonal Anti-Luciferase Antibody (Luci 21 1-107) [PE/Cy7]. Validated: Flow. Tested Reactivity: Firefly. 100% Guaranteed. Antibody [ARG23188] anti-Luciferase antibody [LUC-Y] by Arigo Biolaboratories

    Luciferase antibody | Western | GW20091F | Sigma-Aldrich

    Recombinant luciferin 4-monooxygenase (Luciferase) Application Anti-Luciferase antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200. Physical form Solution in phosphate buffered saline containing 0.02% sodium azide. Abeomics provides Caspase-3, DNMT1, B7-H4, PD-L1 Monoclonal antibodies, ELISA kits, CTLA4 stable cell lines, recombinant proteins, TCF, TLR, Stat reporter cell lines, inhibitors and ligands. ABEOMICS develops Caspase-3, B7-H4, Dnmt1 Antibodies, PD-L1, TLR, TCF Stat Reporter Cell Line CTLA4, PD-1 Stable Cell lines, Luciferase Assay Kit, PD-L1 Pre-Coated ELISA Kits, Recombinant Antibodies ...

    Anti-Luciferase Antibody Products | Biocompare.com

    2 reviews. Compare Anti-Luciferase Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. What is immunohistochemistry… Immunohistochemistry (IHC) is a favorite tool amongst clinicians to help diagnose a range of diseases by identifying abnormal cells, such as those in cancer. In a nutshell, IHC uses antibodies to detect proteins (antigens) that are specific to, or have altered expression in, abnormal cells within a tissue section (for example the liver, pancreas or…

    Anti-Firefly Luciferase antibody (ab21176) | Abcam

    The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. An Alexa-Fluor ® 555 conjugated goat anti-rabbit antibody was used as the secondary. The image shows firefly luciferase (red) in mouse tumour cells and cell nuclei counterstained with Hoechst (blue). See Abreview Detection of GFP in HEK293 Human Cell Line Transfected with GFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) wild-type GFP or (B) eGFP was stained with Goat Anti-GFP APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC4240A). With wide varieties of antibodies, fluorophores, and instruments available for multicolor flow cytometry, optimizing your multicolor experiments can be confusing and frustrating. Use our Multicolor Staining Guide to assist you in developing and optimizing your flow cytometry experiments. BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical ...

    Luciferase Assay - AbVideo™ - Support - Abnova

    Firefly luciferase is widely used as a reporter to study gene expression and other cellular events. The Luciferase Assay is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Through the assay, light is produced by converting the chemical energy of luciferin oxidation and can be measured by a luminometer. Luciferase is a 61 kDa luminescent enzyme isolated from Photinus pyralis (North American Firefly). The enzyme catalyzes the ATP dependent oxidation of beetle luciferin to produce inorganic phosphate and light. Luciferase is frequently used as a reporter protein to quantify expression levels from promoters in tissue culture and in vivo.

    anti-Luciferase antibody | Product No. ABIN1385113

    Rabbit Polyclonal Luciferase antibody for IF (p), IHC (p). Order anti-Luciferase antibody ABIN1385113. Luciferase, iRFP, eGFP, NIS Cell Lines. 4T1 Mammary Carcinoma; ... Immunofluorescence staining for human NIS expression in Mel624-hNIS cells ... This antibody VJ2 detects the native form of NIS and does not recognize the denatured form. The NIS monoclonal antibody clone VJ2 is suitable for flow cytometry and immunofluorescence studies of both ...

    Anti-Firefly Luciferase 抗体 (ab21176) | アブカム

    ab21176 at 10 µg/mL staining Luciferase in transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells by ICC/IF. The cells were fixed with methanol and acetone. An FITC conjugated anti-Rabbit IgG was used as the secondary antibody. Left panel: Un-transfected cells. Right panel: Transfected cells. Rabbit Anti-Bacterial luciferase Antibody. Rabbit Anti-Bacterial luciferase Antibody is available from Creative Biolabs. Home; ... FC Flow Cytometry. FuncS Functional Assay. GS Gel Super Shift Assay. HA Hemagglutination. IA Immunoassay. ... ICFC Intracellular Staining for Flow Cytometry.

    Flow Cytometry Accessory Products | Biotium

    See below for an array of accessory products for use in your flow cytometry experiments: PBS buffer, fixation and permeabilization solutions, and mini cell scrapers to dissociate adherent cells. Visit our other product categories to fulfill your flow cytometry needs: labeled primary antibodies, labeled secondary antibodies, antibody labeling kits, dead cell stains, and cell proliferation dyes. Immunohistochemistry / IHC Antibody-Cell nucleus staining. Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides ...

    Firefly luciferase Antibody (MA1-16880)

    Invitrogen Anti-Firefly luciferase Monoclonal (Luci 21 1-107), Catalog # MA1-16880. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Luciferase samples. Supplied as 100 µL purified antibody. Flow Cytometry Service. RayBiotech is proud to launch our Flow Cytometry Full Testing Service here at our new core facility! Fluorescence-activated Cellular Staining is an ever expanding focus in the Biological Sciences.



    Invitrogen Anti-Firefly luciferase Monoclonal (Luci17), Catalog # MA1-12556. Tested in Western Blot (WB) and Immunohistochemistry (Frozen) (IHC (F)) applications. This antibody reacts with Luciferase samples. Supplied as 100 µg purified antibody (1 mg/mL). FAQs for Luciferase Antibody (NB100-1677). (Showing 1 - 2 of 2 FAQs). One of our customers would like additional information about your goat anti-luciferase (catalog #NB100-1677). They want to use this antibody for immunohistochemistry of paraffin-embedded sections, and would like to know if you recommend an antigen retrieval step? The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. An Alexa-Fluor ® 555 conjugated goat anti-rabbit antibody was used as the secondary. The image shows firefly luciferase (red) in mouse tumour cells and cell nuclei counterstained with Hoechst (blue). See Abreview nvidia linux driver download readme. Flow cytometric analysis of HEK293 cells expressing Firefly Luciferase, staining Firefly Luciferase with ab16466. Cells were fixed with paraformaldehyde at room temperature for 20 min, rinsed twice with permeabilization buffer (PBS, 1% FBS, 0.1% saponin), and incubated with primary antibody. SIGN IN WITH SOCIAL ACCOUNT. Please sign in using your account on one of the following social networks. Select Luciferase antibodies from monoclonal antibodies listed below. View detailed Luciferase antibody specifications by linking to the specific product blocks. Select appropriate Luciferase antibodies for your research by isotype, epitope, applications and species reactivity. To the left of the product names in the table below, 2 reviews. Compare Anti-Luciferase Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. A novel luciferase-based assay for the detection of Chimeric Antigen Receptors . In contrast to Protein-L or anti-idiotype antibody based flow-cytometry assays, the Topanga assay is an assay of . Invitrogen Anti-Firefly luciferase Monoclonal (Luci 21 1-107), Catalog # MA1-16880. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Luciferase samples. Supplied as 100 µL purified antibody. Cornell soccer tournament. Extracellular Staining for Flow Cytometry There are many protocols for staining cells for flow cytometry. Protocols may to need be optimized for different cell types, targets, or applications. This is our basic protocol for extracellular staining of cell surface epitopes in suspension cells for flow cytometry. For intracellular staining, see our Protocol: Intracellular Antibody Staining for . Recombinant luciferin 4-monooxygenase (Luciferase) Application Anti-Luciferase antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200. Physical form Solution in phosphate buffered saline containing 0.02% sodium azide.

    43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73

    Luciferase Antibody Staining For Flow © 2020 Invitrogen Anti-Firefly luciferase Monoclonal (Luci 21 1-107), Catalog # MA1-16880. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin)